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- Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA
- With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes
- The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase
- The resulting plasmid can be then transformed into any competent
- The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases
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